COMPARISON OF APOPTOSIS IN CONTRALATERAL RENAL TUBULAR CELLS IN ORYCTALAGUS CUNICULUS DUE TO ARTIFICIAL UNILATERAL URETERAL OBSTRUCTION, WITH AND WITHOUT VERAPAMIL

Djoyo M Boetoro, Soetojo Soetojo, Doddy M Soebadi, Endang Joewarini, Widodo J P

Abstract


Objective: To compare the effects of verapamil on the contralateral kidney affected by unilateral ureter obstruction. Material & method: The right ureter of oryctalagus cuniculus rabbits, were obstructed surgically with silk suture material, and kept alive for 14 days. One group was obstructed without other treatment, one group was given verapamil from day 7 until day 14 of the obstruction. One group was obstructed and given verapamil from day 0 through day 14. One group was given a sham operation as a control group. The contralateral kidney of all groups were harvested and processed with ApopTag. The increase of apoptotic tubular cells compared to the control group was then analyzed between each group. Results: The highest increase in apoptotic cells was in the obstructed group without verapamil, but increased significantly compared to control (p<0,001). The groups which received verapamil had lower increase of apoptotic tubular cells, in the group given verapamil for 14 days lower than the group which received verapamil only for 7 days. Both the 7 to 14 and the 0 to 14 groups were significantly lower than the group without verapamil (p=0,035 and p<0,001 respectively). Conclusion: verapamil has a protective effect on the contralateral kidney by inhibiting apoptosis caused by unilateral ureter obstruction. While the definitive treatment for urinary obstruction is to relieve it, verapamil can protect the kidney in the mean time.


Keywords


Obstructive uropathy; verapamil; apoptosis

Full Text:

PDF

References


Soebadi DM . Obstruksi saluran kemih atas. In: Basic Science on Urology. Pertemuan Ilmiah Berkala Proyek Trigonum Plus XIII. Malang: FK Unibraw/RSUD Dr. Syaiful Anwar; 2002. p. 1-6.

Tanago EA. Urinary obstruction and stasis. In: Smith’s General Urology, 17th ed. Appleton and Lange, A Publishing Division of Prentice Hall; 2008. p. 165-78.

Gillenwater JY. The pathophysiology of urinary tract obstruction. In: Campbell’s Urology, 8th ed. Philadelphia, London, Toronto, Montreal, Sydney, Tokyo: WB. Saunders Company. 2007; 1: 499-532.

Chevalier. Unilateral ureteral obstruction in early development renal growth: Dependence on duration of obstruction. American Urological Association, Inc. J. Urol 1999; 161 (1): 309-13.

Power R. Mechanical deformation induced apoptosis in human proximal renal tubular epithelial cells is caspase dependent. American Urological Association, Inc. J. Urol 2004; 171 (1): 457-61.

Nicholas JH, Fitzgerald H. Upper urinary tract obstruction. In: The Scientific Basis of Urology, 2nd ed. Oxford: Isis Medical Media; 2004. p. 129-39.

Lama. Pelviureteral junction obstruction: Correlation of renal cell apoptosis and differential renal function. American Urological Association, Inc. J. Urol 2003; 169 (6): 2335-8.

Dash P. Apoptosis. www.sgul.ac.uk/dept/immunology/dash~. Basic Medical Sciences St.George's, University of London; 2007.

Topcu SU, Celik S, Erturhan S, Erbagci A, Yagci F, Ucak R. Verapamil prevents the apoptotic and hemodynamic changes in response to unilateral ureteral obstruction. The Japanese Urological Association, Inc. International Journal of Urology 2008; 15: 350-5.

Burke TJ, Arnold PE, Gordon P, Bulger RE, Dobyan DC, Schrier R. Protective effect of intrarenal calcium membrane blockers before or after renal ischemia. The American Society for Clinical Investigation, Inc. J. Clin. Invest 1984; 74: 1830-41.

Stoneking JE, Hunley TE, Nishimura H, Ji Ma, Fogo A, Inagami T. Renal angiotensin converting enzyme promotes renal damage during ureteral obstruction. American Urological Association, Inc. J. Urol 1998; 160: 1070-4.

Adi PT, Soebadi DM, Soetojo, Sudiana IK, Widodo JP. Ligasi ureter unilateral menyebabkan apoptosis dan proliferasi sel tubulus dan fibroblas di ginjal kontralateral pada kelinci. Indonesian Journal of Urology/Jurnal Urologi Indonesia (JURI) 2007; 14 (1): 5-9.

Syahrir S, Soetojo, Santoso A, Joewarini E, Widodo JP. Apoptosis in ipsilateral kidney: comparison between group receiving verapamil and control group post-artificial total unilateral ureteral obstruction. Indonesian Journal of Urology/Jurnal Urologi Indonesia (JURI) 2010; 17 (1): 9-16.


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.