Objective: Smoking are known to have detrimental effect on sperm. The effects are suspiciously come from nicotine as its main pharmacologic component. This study is carried out to analyze the effect of nicotine exposure per inhalation to the difference of motile sperm percentage and normal morphology sperm percentage of male wistar rats. Material & Methods: Thirty male rats were used for this research. They were randomly divided into 5 groups and treated with nicotine inhalation 0.5 mg/kgBW, 1 mg/kgBW, 2 mg/kgBW and 4 mg/kgBW for 30 days while the control rats were not given any treatment. At the end of experiment, sperm analysis was carried out to measure the percentage of motile sperm and percentage of sperm with normal morphology. Results: Percentage of motile sperm significantly decreased (p<0.05) in all treatment groups compared to control group. Comparison between treatment groups revealed that the percentage of motile sperm significantly decreased in 0.5 mg/kgBW vs 2 mg/kgBW, 0.5 mg/kgBW vs 4 mg/kgBW, and 1 mg/kgBW vs 4 mg/kgBW. Percentage of normal morphology sperm decreased significantly (p<0.05) in 1 mg/kgBW, 2 mg/kgBW, and 4 mg/kgBW compared to control groups. Comparison between treatment groups revealed that there were no significant decreased between each treatment groups. Conclusion: This research conclude that nicotine exposure per inhalation can deteriorate the percentage of motile sperm and the percentage of normal morphology sperm of male wistar rats.
Nicotine, sperm morphology, sperm motility
Centers for Disease Control and Prevention. Current cigarette smoking among adults. United States, 2005–2015. Morbidity and Mortality Weekly Report. 2016; 65(44): 1205–11.
Riset Kesehatan Dasar. Departemen Kesehatan Republik Indonesia; 2013.
Kovac JR, Khanna A, Lipshultz LI. The effects of cigarette smoking on male fertility. Postgrad Med. 2015; 127(3): 338–41.
Ramlau-Hansen CH, Thulstrup AM, Aggerholm AS, Jensen MS, Toft G, Bonde JP. Is smoking a risk factor for decreased semen quality? A cross-sectional analysis. Hum Reprod. 2007; 22: 188–96.
Harlev A, Agarwal A, Gunes SO, Shetty A, Simon du Plessis S. Smoking and male infertility: An Evidence-Based Review. World J Mens Health. December 2015; 33(3): 143-60.
Chia SE, Xu B, Ong CN, Tsakok FM, Lee ST. Effect of cadmium and cigarette smoking on human semen quality. Int J Fertil Menopausal Stud. 1994; 39: 292-8.
Colagar AH, Jorsaraee GA, Marzony ET. Cigarette smoking and the risk of male infertility. Pak J Biol Sci. 2007; 10: 3870-4.
Richthoff J, Elzanaty S, Rylander L, Hagmar L, Giwercman A. Association between tobacco exposure and reproductive parameters in adolescent males. Int J Androl. 2008; 31: 31-39.
Oyeyipo IP, Raji Y, Emikpe BO, Bolarinwa AF. Effects of nicotine on sperm characteristics and fertility profile in adult male rats: a possible role of cessation. J Reprod Infertil. 2011; 12: 201–7.
Wong WY, Thomas CM, Merkus HM, Zielhuis GA, Doesburg WH, Steegers-Theunissen RP. Cigarette smoking and the risk of male factor subfertility: minor association between cotinine in seminal plasma and semen morphology. Fertil Steril. 2000; 74: 930–5.
Aydos K, Given MC, Can B, Ergun A. Nicotine toxicity to the ultra-structure of the testis in rats. BJU Int. 2001; 88: 622-6.
Jana K, Samanta PK, De DK. Nicotine diminishes testicular gametogenesis, steroidogenesis, and steroidogenic acute regulatory protein expression in adult albino rats: possible influence on pituitary gonadotropins and alteration of testicular antioxidant status. Toxsci. 2010; 116(20): 647-59.
Mosadegh M, Hasanzadeh S, Razi M. Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression. Iran J Basic Med Sci. 2017; 20(2): 199–208.
Schill WB, Comhaire FH. Andrology for the Clinician. Springer; 2006.
Kalikauskas V, Blaustein D, Ablin RJ. Cigarette smoking and its possible effects on sperm. Fertil Steril. 1985; 44: 526-8.
Patterson TR, Stringham JD, Meikle AW. Cotinine and nicotine inhibit steroidogenesis in mouse Leydig cells. Life Sci. 1990; 46(4): 265-72.
Patterson TR, stringham JD, Meikle AW. Nicotine and cotinine inhibit steroidogenesis in mouse leydig cells. Life Sci. 1990; 46: 265-72.
Saleh RA, Agarwal S. Oxidative stress and male infertility: From Research Bench to Clinical Practice. Journal of Andrology. 2002; 23(6): 737-52.
Duru NK, Morshedi M, Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertilility and Sterility. 2000; 74(6): 1200-7.
Elshal MF, El-Sayed IH, Elsaied MA, El-Masry SA, Kumosani TA. Sperm head defects and disturbances in spermatozoal chromatin and DNA integrities in idiopathic infertile subjects: Association with cigarette smoking. Clinical Biochemistry. 2009; 42: 589-94.
Rubes J, Lowe X, Moore D, Perreault S, Slott V. Smoking cigarettes is associated with increased sperm disomy in teenage men. Fertility and Sterility. 1998; 70(4): 715-23.
Sharma R. Smoking and semen quality: A new meta-analysis examining the effect of the 2010. World Health Organization Laboratory Methods for the Examination of Human Semen. Eur Urol; 2006. http://dx.doi.org/10.1016/j.eururo.2016.04.010
Cooke MS, Evans MD, Dizdaroglu M, Lunec J. Oxidative DNS damage: mechanisms, mutation, and disease. The FASEB Journal. 2003; 17: 1195-214.
Asiyah HA, Syazana NS, Hashida NH, Durriyyah Sharifah HA, Kamaruddin MY. Effects of nicotine and Gelam honey on testis parameters and sperm qualities of juvenile rats. Scientific Research and Essays. 2011; 6(26): 5471-4.
Shaker Ali S, Hamed EA, Ayoub NN, Shaker Ali A, Suliman MI. Effects of different routes of nicotine administration on gastric morphology and hormonal secretion in rats. Exp Physiol. 2015; 100(8): 881–95.
Matta SG, Balfour DJ, Benowitz NL, Boyd RT, Buccafusco JJ. Guidelines on nicotine dose selections for in vivo research. Psychopharmacology. 2007; 190: 269–319.
Oyeyipo IP, Obembe OO, Oladokun OO, Raji Y. Sperm function and fertility profile following nicotine administration in male rats: Protective potentials of Zingiber officinale. Int J Green Pharm. 2014; 8: 125-9.
Yamamoto Y, Isoyama E, Sofikitis N, Miyagawa I. Effects of smoking on testicular function and fertilizing potential in rats. Urol Res. 1998; 26: 45–48.
Oyeyemi WA, Shittu ST, Kolawole TA, Ubanecheand P, Akinola AO. Protective effect of vitamin E on nicotine induced reproductive toxicity in male rats. Nigerian Journal of Basic and Applied Science. 2015; 23(1): 7-13.
Condorelli RA, La Vignera S, Giacone F, Iacoviello L, Vicari E. In-vitro effects of nicotine on sperm motility and bio-functional flow cytometry sperm parameters. Int J Immunopathol Pharmacol. 2013; 26(3): 739-46.