Objective: To analyze the difference in the number of spermatogonia, leydig cells and sertoli cells in young age of white mice Wistar strain after inhalation of chronic nicotine exposure. Material & Method: Laboratory experimental study with post test only control group design, measurement of spermatogonium, leydig cell, sertoli cell in 5 groups of young male Wistar strain, negative control group and treatment group given nicotine exposure 0.5 mg, 1 mg, 2 mg, and 4 mg/kg body weight/day for 30 days. Results: A significant reduction in spermatogonium was found in the group given nicotine 0.5 mg/kgBW/day (p=0.048), 1 mg/kgBW/day (p=0.002), 2 mg/kgBW/day (p=0.002) and 4 mg/kgBW/day (p=0.000) when compared to the control group. Significant decreases were also seen in the group receiving 4 mg of nicotine exposure compared with 0.5 mg (p=0.018). Significant decrease in sertoli cell count was seen only in the nicotine group of 4 mg/kgBW/day compared with the control group (p=0.047). A significant decrease in leydig cell count was found in the nicotine 2 mg/kgBW/day (p=0.037) and nicotine group 4 mg/kgBW/day (p=0.023) when compared with the control group. Significant decreases were also found in the 4 mg/kgBW/day group compared to the 0.5 mg/kgBW/day group (p=0.004). In this study there were also a decrease in the number of spermatogonia, sertoli cells, and leydig cells in the increased dose of nicotine given although not statistically significant. Conclusion: Chronic exposure of nicotine per inhalation may decrease the number of spermatogonia, sertoli cells, and leydig cells. The higher the dose of nicotine given the greater the decrease in the number of spermatogonium cells, sertoli cells, and leydig cells that occur. This proves that nicotine is one of the causes of infertility in men.
Nicotine, spermatogonia, sertoli cells, leydig cells, infertility
Jungwirth A, Diemer T, Kopa Z, Krausz C, Minhas S, Tourmaye H. EAU guidelines on male infertility. Europeam Association of Urology; 2018.
Indonesian Urological Association. Panduan penanganan infertilitas pria; 2015.
Agarwal A, Said TM. Oxidative stress, DNS damage, and apoptosis in male infertility. BJU International; 2005. p. 95.
Vinodini N, Nayanatara A, Damodara G, Ahamed B, Ramaswamy C, Shabarinath & Bhat R. Effect of monosodium glutamat-induced oxidative damage on rat testis. Journal of Chinese Clinical Medicine. 2008; 3: 370-3.
Moreno G, Perello M, Gaillardand RC, Spine E. Orexin a stimulates hypothalamic- pituitary-adrenal (HPA) axis function, but not food intake in the absence of full hypothalamic NPYergic activity. Endocrine. 2005; 26: 99–106.
Pavlovic V, Kocic D, Sokolovic D. Effect of monosodium glutamate on oxidative stress and apoptosis in rat thymus. J Mol Cell Biochem. 2007;303:161–6.
World Health Organization. WHO report on the global tobacco epidemic. The MPOWER Package; 2008. Available from: http://www.who.int/tobacco/2. mpower/mpower_report_full_2008_pdf
Oyeyipo IP, Raji Y, Emikpe BO, Bolarinwa AF. Effects of oral adminmistration of nicotine on organ weight, serum testosteron level and testicular histology in adult male rats. Nig J Physiol Sci. 2010; 25: 81-86.
Dahlan S. Statistik untuk kedokteran dan kesehatan: uji hipotesis. Jakarta: Bina Mitra Press; 2006.
Snedecor, Cochran. Ten thousand randomly assorted digits. In: Statistic Methods. Iowa: The Iowa State University Press; 1974. p. 543.
Mendelson JH, Scholar MB, Mutshler NH, Jaszyma-Gasior M, Goletiami NV, Siegel AJ, et al. Effects of intravenous cocaine and cigarette smoking on leuteinizing hormone, testosterone, and prolactin on men. J Phamacol Exp Therap. 2003; 308: 339–48.
Oates JA. Pharmacologic aspects of cigarette smoking and nicotine addiction. Nejm; 1988.
Latha MS, Vijayammal PL, Kurup PA. Effect of nicotine administration on lipid metabolism in rats. Indian Journal of Medical Research. 1993; 98: 44–49.
Ashakumary L, Vijayammal PL. Additive effect of alcohol and nicotine on LPO and antioxidant defence mechanism in rats. Journal of Application Toxicology. 1996; 16: 305–8.
Bui LM, Keen CL, Dubick MA. Comparative effect of 6 week nicotine treatment on blood pressure and components of the antioxidant system in male spontaneously hypertensive and normotensive wistar kyoto rats. Toxicology. 1995; 98: 57-65.
Yildiz D, Ercal N, Armstrong DW. Nicotine enantiomers and oxidative stress. Toxicology. 1998; 130: 155-65.
Slater TF. Free radical mechanisms in tissue injury. Biochemistry Journal. 1984; 222: 1-8.
Helen A, Krishnakumar K, Vijayammal PL, Augusti KT. Antioxidant effect of onion oil (Allium cepa. Linn) on the damages induced by nicotine in rats as compared to alpha-tocopherol. Toxicology Letters. 2000; 116: 61-68.
Mahanem MN, Nor-Asmaniza AB, Phang HT. Effects of nicotine and co-administration of nicotine and vitamin E on testis and sperm quality of adult rats.
Autifi AHM, Elsagheir AS, Almandouh MAL. Effect of nicotine on the testis of adult albino rat and the possible protective effect of vitamin E. Nat Sci. 2017; 15(4): 13-19.
Reddy S, Londonkar R, Ravindra Reddy Somnath, Patil SB. Testicular changes due to graded doses of nicotine in albino mice. Indian J Physiol Pharmacol. 1998; 42(2): 276-80.
Anderson K, Fuxe K, Eneroth P, Agnati LF. Involvement of cholinergic nicotine like receptors as modulators of amine turn over in various terminal systems and prolactine, LH, FSH & ICSH secretion in casstrated male rats. Acta Physiologica Scandinavia. 1982; 116: 41-50.
Calvalho CAF, Favaro WJ, Padovani CR, Cagnon VHA. Morphometric and ultrastructure features of the ventral prostate of rats (rattus norvegicus) submitted to long-term nicotine treatment. Andrologia. 2006; 38: 142-51.
Leson CR, Leeson TS, Paparo AA. Textbook of histology. Alih bahasa: Tambayong J. Jakarta: Penerbit Buku Kedokteran EGC; 1996. p. 515-7.
Fawcett DW. A textbook of histology, 12/E. Alih bahasa: Tambayong J. Jakarta: Penerbit Buku Kedokteran EGC; 2002. p. 687-96.
Nesseim WH, Haroun HS, Mostafa E, Youakim MF, Mostafa T. Effect of nicotine on spermatogenesis in adult albino rats. Andrologia. 2011; 43: 398-404.
Jana K, Samanta PK, De DK. Nicotine diminishes testicular gametogenesis, steroidogenesis, and steroidogenic acute regulatory protein expression in adult albino rats: possible influence on pituitary gonadotropins and alteration of testicular antioxidant status. Toxsci. 2010; 116(20): 647-59.
De Kretser, Kerr JB. The cytology of testis. In: Knobil & JD Neil (eds.) The Physiology of Reproduction. Vol. I. Raven Press: New York; 1988.
Kim KH, Joo KJ, Park HJ, Kwon CH, Jang MH, Kim CJ. Nicotine induces apoptosis in TM3 mouse leydig cells. Fertility and Sterility. 2005; 82: 1093-9.
Kanwal S, Ameer MK, Mehboob F. Toxic effect of nicotine on leydig cell count and testosteron levels in adult albino mice and its protection by date palm pit powder.
Rotenberg KS, Adir J. Pharmacokinetics of nicotine in rats after multiple cigarette smoke exposure. Toxicology and Applied Pharmacology. 1983; 69: 1-11, 28.
Rotenberg KS, Miller RP, Adir J. Pharmacokinetics of nicotine in rats after single cigarette smoke inhalation. Journal of Pharmaceutical Sciences. 1980; 69: 1087-90.