DIFFERENCES OF TESTOSTERONE LEVELS TO THE DURATION OF INHALED NICOTINE EXPOSURE IN SPRAGUE-DAWLEY MALE WHITE RATS
AbstractObjective: To analyze the differences in testosterone level in young Sprague-Dawley white rats after exposure of inhaled nicotine and the recovery effect that occurs when nicotine exposure is stopped. Material & Methods: This is an experimental study, which variable measurements were taken after the experimental animals were treated. The experimental grouping process was carried out randomly, with repetitions of 12 experimental animals in each group and the control group as a comparison (negative control). This study using male Sprague-Dawley white rats as samples. Nicotine administration was carried out by inhalation using pure nicotine (C10H14N2), which was diluted using distilled water. Treatment group 1 (P1) was given nicotine exposure for 30 days, treatment group 2 (P2) for 15 days, and treatment group 3 (P3) for 15 days and then stopped for 15 days compared to the control group. Testosterone levels are measured using the ELISA (Enzyme-Linked Immunosorbent Assay) method in the morning. Results: The lowest testosterone levels were found in the P1 and significantly different from the P2 (p=0.029) and P3 (p=0.001). The results of the analysis show that a decrease in testosterone levels in mice is related to the duration of nicotine exposure. Conclusion: Testosterone levels in the control group were significantly higher compared to the treatment group that exposed by nicotine inhalation with a dose of 4 mg/kgBW/day for 15 days, and also there is a significant recovery effect on testosterone levels after the treatment was halted for 15 days.
Benowitz NL. Pharmacologic aspects of cigarette smoking and nicotine addiction. N Engl J Med. 1988; 312: 1318–30.
Agarwal A, Said TM. Oxidative stress, DNA damage and apoptosis in male infertility: A clinical approach. BJU Int. 2005; 95(4): 503–7.
Balai Pengujian Hewan. Teknis pemeliharaan hewan percobaan untuk pengujian mutu obat hewan. Jakarta: Departemen Pertanian; 1989. p. 8–11.
Suresh Sikka, Wayne Hellstrom. Bioenvironmental Issues Affecting Men's Reproductive and Sexual Health 1st Edition. Academic Press Elsevier: USA; 2017. p. 487.
Mooradian AD, Morley JE, Korenman SG. Biological actions of androgens". Endocrine Reviews. 1987; 8(1): 1–28.
Weinbauer G., Luetjens C., Simoni M, Nieschlag E. Physiology of testicular function. In: Nieschlag E, Behre H., Nieschlag S, editors. Andrology : Male Reproductive Health and Dysfunction. Berlin: Springer; 2010. p. 11–60.
Benowitz NL, Hukkanen J, Jacob III P. Nicotine Chemistry, Metabolism, Kinetics and Biomarkers. Handb Exp Pharmacol. 2009; 192: 29–60.
Tweed JO, Hsia SH, Lutfy K, Friedman TC. The endocrine effects of nicotine and cigarette smoke. Trends Endocrinol Metab. 2012; 23(7): 334–42.
Mosadegh M, Hasanzadeh S, Razi M. Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression. Iran J Basic Med Sci. 2017; 20(2): 199–208.
Oyeyipo I, Raji Y, Bolarinwa A. Antioxidant profile changes in reproductive tissues of rats treated with nicotine. J Hum Reprod Sci. 2014; 7(1): 41.
Asadi N, Bahmani M, Kheradmand A, Rafieian-Kopaei M. The impact of oxidative stress on testicular function and the role of antioxidants in improving it: A review. J Clin Diagnostic Res. 2017; 11(5): IE01–5.
Oyeyipo IP, Raji Y, Emikpe BO, Bolarinwa AF. Effects of oral administration of nicotine on organ weight, serum testosterone level and testicular histology in adult male rats. Niger J Physiol Sci. 2010; 25(1): 81–6.
Oyeyipo IP, Raji Y, Bolarinwa AF. Nicotine alters male reproductive hormones in male albino rats: The role of cessation. J Hum Reprod Sci. 2013; 6(1): 40–4.
Aprioku JS, Ugwu TC. Tobacco smoke exposure induces irreversible alteration of testicular function in prepubertal rats. J Basic Clin Physiol Pharmacol. 2016; 27(6): 577–84.
Guo X, Wang H, Wu X, Chen X, Chen Y, Guo J, et al. Nicotine affects rat Leydig cell function in vivo and vitro via down-regulating some key steroidogenic enzyme expressions. Food Chem Toxicol. 2017; 110: 13–24.
Rizaldi A. Pengaruh paparan kronis nikotin secara inhalasi terhadap jumlah sel spermatogonium, sel sertoli, dan sel leydig tikus putih strain wistar usia muda. Universitas Airlangga; 2018.
Zhao, J. et al. Cigarette smoking and testosterone in men and women : A systematic review and meta-analysis of observational studies. Preventive Medicine. Elsevier Inc; 2016. p. 1–10.
Turki, H. A. Al. Effect of smoking on reproductive hormones and semen parameters of infertile Saudi Arabian. 2015; 7(1).
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