Published 2021-01-15
Ahmad Zulfan Nickanor K. R Wonatorey


Objective: This study aims to analyze the effects of hyperglycemia status on the function of testicular spermatology, especially CAT-1. Material & Methods: This study was an experimental pre-clinical study. Twenty-seven rats were divided into 3 groups: normal, 2 weeks, and 4 weeks hyperglycemia. The hyperglycemic state in the Wistar rats was induced by Streptozotocin (STZ). All data were collected and analyzed with SPSS 20.0. At two and four weeks, testicular tissue was extracted and it will be processed using the Total RNA Mini Kit FavorPrepTM then quantitative PCR was performed using the SYBR® Fast qPCR Kit. Results: CAT-1 gene expression in the hyperglycemia induction group increased when induced for 2 weeks and again after 4 weeks compared to controls (18.88 ± 4.7 and 21.45 ± 5.52 vs 10.83 ± 3.4). However only induction after 2 weeks was statistically significant (p= 0.021). Conclusion: CAT-1 (Catalase) gene expression has increased in testicular tissue under conditions of hyperglycemia.



CAT-1, hyperglycemia, DM, infertility, gene expression


NIH Consensus Conference. Impotence. NIH Consensus Development Panel on Impotence. JAMA. 1993; 270(1): 83-90.

van Kollenburg RAA, de Bruin DM, Wijkstra H. Validation of the Electronic Version of the International Index of Erectile Function (IIEF-5 and IIEF-15): A Crossover Study. J Med Internet Res. 2019; 21(7): e13490.

Wagner H, Cheng JW, Ko EY. Role of reactive oxygen species in male infertility: An updated review of literature. Arab J Urol. 2018; 16(1): 35-43.

Yan X, Dong L, Liu Y, et al. Effects of physical exercises on semen quality and reproductive outcomes in male infertility: A protocol for systematic review and meta-analysis of randomized controlled trials. Medicine (Baltimore). 2019; 98(41): e17494.

Rubio-Riquelme N, Huerta-Retamal N, Gómez-Torres MJ, Martínez-Espinosa RM. Catalase as a molecular target for male infertility diagnosis and monitoring: An overview. Antioxidants. 2020; 9(1): 1-18.

Hodgkinson AD, Bartlett T, Oates PJ, Millward BA, Demaine AG. The response of antioxidant genes to hyperglycemia is abnormal in patients with type 1 diabetes and diabetic nephropathy. Diabetes. 2003; 52(3): 846-851.

Tremellen K. Oxidative stress and male infertility--a clinical perspective. Hum Reprod Update. 2008; 14(3): 243-258.

Yu B, Huang Z. Variations in Antioxidant Genes and Male Infertility. 2015; 2015.

Tabatabaei-Malazy O, Khodaeian M, Bitarafan F, Larijani B, Amoli MM. Polymorphisms of antioxidant genes as a target for diabetes management. Int J Mol Cell Med. 2017; 6(3): 135-147.

Ornoy A, Livshitz A, Ergaz Z, Stodgell CJ, Miller RK. Hyperglycemia, hypoxia and their combination exert oxidative stress and changes in antioxidant gene expression: studies on cultured rat embryos. Birth Defects Res Part B Dev Reprod Toxicol. 2011; 92(3): 231-239.

Patel H, Chen J, Das KC, Kavdia M. Hyperglycemia induces differential change in oxidative stress at gene expression and functional levels in HUVEC and HMVEC. Cardiovasc Diabetol. 2013; 12: 142.

Alahmar AT. Role of Oxidative Stress in Male Infertility: An Updated Review. J Hum Reprod Sci. 2019; 12(1): 4-18.

Sabouhi S, Salehi Z, Bahadori MH, Mahdavi M. Human catalase gene polymorphism (CAT C-262T) and risk of male infertility. Andrologia. 2015; 47(1): 97-101.

Del Prete C, Ciani F, Tafuri S, et al. Effect of superoxide dismutase, catalase, and glutathione peroxidase supplementation in the extender on chilled semen of fertile and hypofertile dogs. J Vet Sci. 2018; 19(5): 667-675.

Bisht S, Faiq M, Tolahunase M, Dada R. Oxidative stress and male infertility. Nat Publ Gr. 2017; 14(8): 1-15.

Copyright Information
Department of Urology, Faculty of Medicine/Airlangga University