Objective: To investigate the correlation of follicle stimulating hormone (FSH) and luteinising hormone (LH) with testicular sperm biopsy result in azoospermia patients. Material & methods: This is a cross sectional descriptive analytic study. Data were collected from medical record in Klinik Permata Hati Sardjito General Hospital Yogyakarta. We divided them into two groups, sperm group and non-sperm group of sperm biopsy result. We recorded the level of FSH and LH pre-operation. The results were expressed as mean ± standard deviation (SD). The correlation coefficient (r) between various parameters was determined by analysis for Spearman’s rank correlation test. Results: We found 35 patients who met the inclusion and exclusion criteria during 2010-2012, who performed testicular biopsy, age range between 28-44 yo. The mean of FSH on sperm group (12.75 mIU/mL) was higher than non-sperm group (7.26 mIU/mL). The mean of LH on sperm group (5.8 mIU/mL)was also slightly higher than non-sperm group (5.70 mIU/mL). We found weak correlation between FSH level and testicular biopsy (r = 0.095), while on LH level was found negative correlation with testicular biopsy (r = -0.053). There were 42.85% patients with negative sperm result within normal range of FSH and 100% with normal range of LH. The Level of FSH with positive result ranged between 1.94-19.7 mIU/mL and LH level with positive result were 1.38-17.69 mIU/mL. Conclusion: FSH and LH were important plasma hormones correlated with spermatogenesis. FSH level between 1.94-19.7 mIU/mL and LH level between 1.38-17.69 mIU/mL could be used as reliable criteria for testicular sperm biopsy.Keywords: Follicle stimulating hormone, luteinising hormone, testicular sperm biopsy, azoospermia.
Paul J. Turek, M.D. Male infertility in: Tanagho EA, Mc Aninch JW, editors. Smith’s General Urology. 17th Ed. Conecticut: Mc Graw-Hill; 2008. p. 684-701.
Jungwirth A. Guidelines on Male Infertiliy. European Association of Urology; 2012.
Guyton and Hall. Fungsi reproduksi dan hormonal pria (dari kelenjar pineal). Buku Ajar Fisiologi Kedokteran. Edisi 9. Jakarta: EGC; 1997. p. 1265-77.
Tang WH. Correlation of testicular volume and reproductive hormone level with the result of testicular sperm aspiration in on-obstructive azoospermia patient. Zhounghua Nan Ke Xue. 2012; 18(1): 48-51.
Weiss DB. Follicle-stimulating hormone in azoospermia in preicting of spermatogenenic pattern. Harefuah. 1998; 135(5-6): 169-75, 256.
Reza Moein, M. Relation between histology of testicular biopsy and level of sex hormones in infertile men with non-obstructive azoospermia. Journal of Reproduction and infertility. 2001 July-Sept; 2(3).
Blandy J. Surgery of the testicle. Operatif Urology. London: Blackwell Scientific Publication; 1978. p. 227-8.
Sabanegh A, Agarwal A. Male infertility. In: Campbell-Walsh Urology. 10th ed. Philadelphia: Elsevier Saunders; 2012. p. 617-47.
Sussman EM, Chudnovsky A, Niederberger CS. Hormonal evaluation of the infertile male: has it evolved? Urol Clin N Am. 2008; 35: 147-55.
Ozturk U, Ozdemir E, Dede O. Assessment of anti-sperm antibodies in couple after testicular sperm extraction. Clin Invest Med. 2011; 34: 180-3.
Geidam AD, Yawe KD, Adebayo AE, Idrisa A. Hormonal profile of men investigated for infertility at the University of Maiduguri in Northern Nigeria. Singapore Med J. 2008; 49: 538.
Ziaee SA, Ezzatnegad M, Newroozi M, Jamshidian M, Abdi H, Moghaddam SM. Prediction of successful sperm retrieval in patients with non-obstructive azoospermia. Urology Journal UNRC/IUA. 2006; 3: 92-6.
Massart A, Lissens W, Tournaye H, Stouffs K. Genetic causes of spermatogenic failure. Asian Journal of Andrology, 2012; 14: 40-8.
Moretti E, Pascarelli NA, Gianerini M. 18,X,Y aneuploidies and transmission electron microscopy studies in spermatozoa from five carriers of different reciprocal translocations. Asian Journal of Andrology. 2009; 11: 325-32.
Jhaveri KS, Mazrani WM, Chawla TP. The role of cross-sectional imaging in male infertility: A pictorial review. Canadian Association of Radiologist Journal. 2010; 61: 144-55.
Du J, Li FH, Guo YF. Differential diagnosis of azoospermia and etiologic classification of obstructive azoospermia: role of scrotal and transrectal US. Radiology. 2010; 256: 493-503.