Published 2018-01-11
I Gede Andre Arda Pratama H R Danarto


Objective: To know the effect of purwoceng extract (Pimpinella alpine) on the expression of neuronal nitric oxide synthase (nNOS) in the penile tissue of white male rats (Rattus novergicus) through immunohistochemical examination. Material & Methods: Animal experiments consisted of 27 male rats, three months of age with a mean body weight of approximately 200 grams were divided into three groups randomly, one group consisted of 10 rats. Treatment 1 rats receive Pimpinella alpina extract with 50 mg/ml, Treatment 2 rats receive Pimpinella alpina extract with 100 mg/ml. In the control group rats receive aquadest as much as 1 ml. Results: In the control group the average is 75.67, in the treatment group 1 is 113, and in group 2 is 181.44. The difference in treatment between groups was performed using one-way ANOVA test. Between control and treatment groups 1 the different is significant with p value of 0.013 (p<0.05). The mean expression of nNOS between treatment groups 1 and 2 differ significantly with p=0.00 (p<0.05). Conclusion: Purwoceng extract (Pimpinella alpine) can increase the expression of nNOS on NANC nerve fibers (nonadrenergic noncholinergic) in the corpora cavernosa of the penis that causes smooth muscle relaxation in penile erection.



Purwoceng (Pimpinella alpine), neuronal nitric oxide synthase (nNOS), erection


Ireng Darwati, Ika Roostika. Status penelitian purwaceng. Buletin Plasma Nuftah; 2006: 12(1).

Suzery, Meiny, Cahyono. Produksi senyawa afrodisiak dari purwoceng (Pimpinella alpine Molk): Pengembangan potensi “natural resources” khas Jawa Tengah. Universitas Diponegoro Semarang; 2011.

Otih R. 2nd International Conference and Exhibition on Pharmacognosy, Phytochemistry & Natural Products Pruatjan (Pimpinella pruatjanMolk): The rooted herbal medicine of Indonesia for aphrodisiac properties. Indonesian Agency for Agricultural Research and Development, Indonesia.

Taufiqqurrachman. Pengaruh ekstrak Pimpinella alpine Molk. (Purwoceng) dan akar Eurycomalongifolia Jack. (Pasak bumi) terhadap peningkatan kadar testosterone, LH, dan FSH serta perbedaan peningkatannya pada tikus jantan Spragul Dawley. Tesis, Pascasarjana Ilmu Biomedik. Universitas Diponegoro Semarang; 1999. p. 119.

Nasihun T. Pengaruh pemberian ekstrak purwoceng (Pimpinella alpine Molk) terhadap peningkatan indikator vitalitas pria studi eksperimental pada tikus jantan Sprague Dawley. Sains Medika. Januari – Juni 2009; 1(1).

Suhartinah. Efek spermatogenesis dan aprodisiaka herba purwoceng. Fakultas Farmasi Universitas Setiabudi Surakarta; 2011.

Juniarto AZ. Perbedaan pengaruh pemberian ekstrak eurycoma longifolia dan pimpinella alpine pada spermatogenesis tikus Sprague Dawley. Tesis, Pasca Sarjana Ilmu Biomedik: Universitas Diponegoro Semarang; 2004. p. 63.

Penson DF, Wessells H. Erectile dysfunction in diabetic patients. Diabetes Spectrum. 2004; 17: 225-30.

Brown JS, Wessel H, Chancellor MB, Howards SS, Stamm WE, Stapleton AE, et al. Urologic complications of diabetes: Diabetes care. 2005; 28: 177-85.

El-Sakka AI, Yassin AA. Amelioration of penile fibrosis: Myth or reality. J Androl. 2010; 31: 324-35.

Nestor F, Gonzalez-Cadavid, Arthur, Thomas. Expression of penile neuronal nitric oxide synthase variants in the rat and mouse penile nerves. Biology of Reproduction. 2000; 63: 704-14.

Shabsigh, R. The effects of testosterone on the cavernous tissue and erectile function. World J Urol. 1997; 15: 21–26.

Cashen D, McIntyre, Martin. Effects of sildenafil on erectile activity in mice lacking neuronal or endothelial nitric oxide synthase. Br J Pharmacol. 2002; 136: 693-700.

Mills TM, Reilly CM, Lewis RW. Androgens and penile erection: A review. J Androl. 1996; 17: 633-8.

Morales A, Heaton JP. Hypogonadism and erectile dysfunction: Pathophysiological observations and therapeutic outcomes. BJU Int. 2003; 92: 896-9.

Meisel RL, Sachs BD. The physiology of male sexual behavior. In: Knobil E, Neill JGS, Greenwald GS, Markert CL, Pfaff DW, Ed. The Physiology of Reproduction. New York: Raven Press. 1994; 2: 3-107.

Burnett AL. Neurophysiology of erectile function: Androgenic effects. J Androl. 2003; 24: S2-5.

Saenz de Tejada I. Further commentary: Physiology of erectile function and pathophysiology of erectile dysfunction. In: Lue TF, Basson R, Rosen R, Giuliano F, Khoury S, Montorsi F, Ed. The Second International Consultation on Sexual Medicine: Sexual Medicine-Sexual Dysfunctions in Men and Women. Plymouth: Plymbridge Distributors Ltd; 2004. p. 289-343.

Traish AM, Park K, Dhir V, Kim NN, Moreland RB, Goldstein I. Effects of castration and androgen replacement on erectile function in a rabbit model. Endocrinology. 1999; 140: 1861-8.

Gunardi, S. Anatomi sistem reproduksi. Cetakan ke-2. Jakarta: Balai Penerbit FKUI; 2007.

Tendean OS. Pemeriksaan-pemeriksaan klinis pada pria infertil. Manado: Bagian Biologi/Andrologi: Fakultas Kedokteran Sam Ratulangi; 2004.

Pangkahila W. Disfungsi ereksi. Jakarta: Yayasan Penerbitan Ikatan Dokter Indonesia; 2006.

Noburo, Kazuhede, Tomio. Nitric oxide and penile erectile function, Pharmacology & Therapeutics: Elsevier; 2005.

David F, Chris, Jacob, Nestor. Androgen and pituitary control of penile nitric oxide synthase and erectile function in the rat. Biology of Reproduction. 1996; 55: 567-74.

Raquel, Ana, Pedro, Manuel. Androgen-dependent nitric oxide release in rat penis correlates with levels of constitutive nitric oxide synthase isoenzymes. Biology of Reproduction. 1999; 61: 1012-6.

Wu-Jiang, Zhong, Hua, Yi-Ming. Effects of icariin on erectile function and expression of nitric oxide synthase isoforms in castrated rats. Asya J Androl. 2005; 7(4): 381-8.

Copyright Information
Department of Urology, Faculty of Medicine/Airlangga University