Published 2018-07-26
Ferdyan Rachmat Efendi Johan Renaldo Tarmono Djojodimedjo


Objective: To investigate the effect of dexamethasone on spermatogonium and sertoli cell of ipsilateral testis in unilateral testicular torsion strain wistar rat. Material & Method: Experimental study with post-test only control group design. The present  study was conducted on 30 Wistar male rats aged 10 – 12 weeks grouped into 5 groups. Group I was the normal/sham operation group (KN), group II was left testicular torsion for 4 hours group and followed  by manual detorsion  (K1), group III was left testicular torsion for 10 hours group and followed  by manual detorsion (K2),  group IV was left testicular torsion for 4 hours group and given dexamethasone 10 mg/kg body weight subcutaneously 30 minutes before manual etorsion (D1), and group V was left testicular torsion for 10 hours group and  given dexamethasone 10 mg/kg body weight subcutaneously 30 minutes before manual detorsion. All rats had left orchidectomy 4 hours after detorsion. The number of spermatogonium and sertoli cells were counted in histological seminiferous tubular testis that have obtained Haematoxylin Eosin staining. Data were analyzed by ANNOVA followed by Post Hoc Tukey for spermatogonium and Kruskal Wallis followed by Mann Whitney test for sertoli cell. Differences were considered significant at p <0.05. Results: There was significant difference in the mean number of spermatogonium between K1 & D1 group. Otherwise, there was no significant difference in the mean number of spermatogonium between K2 & D2. There was significant difference in the mean number of Sertoli cells between K1 & D1 group, likewise that between K2 & D2 group. Conclusion: These results suggest that dexamethasone has protective effect in spermatogonium and sertoli cell in testicular torsion for 4 hours.



dexamethasone, spermatogonium, sertoli cells, testicular torsion


Feher A, Bajory Z. A review of main controversial aspects of acute testicular torsion. J Acute Dis. 2016; 5(1): 1–8.

Hardjowijoto S. The effects of unilateral testicular torsion upon immunity modulation and apoptosis of germinal cells in the contralateral testis-An experimental study in rats. Folia Medica Indonesiana. 2005; 41(1): 9–17.

Ringdahl E, Teague L. Testicular Torsion. Am Fam Physician. 2006; 74: 1739–43.

Nurhadi P, Soetojo. Profil Akut Skrotum di Rumah Sakit Umum Dr.Soetomo Surabaya Periode Januari 2011-Desember 2015. Retrospective Study; 2015.

Shimizu S, Martin DT, Dimitriadis F, Satoh K, Saito M. The effect of ischemic preconditioning and postconditioning on testicular torsion-detorsion injury. Glob J Biochem. 2011; 3: 1.

Elshaari F, Elfagih RI, Sheriff D, Barassi I. Cytology & Histology Testicular Torsion-Detorsion- Histological and Biochemical Changes in Rat Testis. J Cytol Histol. 2012; 3(1): 1–6.

Krarup T. The Testes After Torsion. Br J Urol. 1978; 50: 43–6.

Lysiak JJ, Turner SD, Nguyen QAT, Singbartl K, Ley K, Turner TT. Essential Role of Neutrophils in Germ Cell-Specific Apoptosis Following Ischemia/Reperfusion Injury of the Mouse Testis. Biol Reprod. 2001; 725: 718–25.

Turner TT, Tung KSK, Tomomasa H, Wilson LW, Al TET. Acute Testicular Ischemia Results in Germ Cell-Specific Apoptosis in the Rat. Biol Reprod. 1997; 1274: 1267–74.

Vigueras RM, Reyes G, Rojas P, Hernández R. Testicular torsion and its effects on the spermatogenic cycle in the contralateral testis of the rat. Lab Anim. 2004; 38: 313–20.

Sharp VJ, Arlen AM. Testicular Torsion: Diagnosis, Evaluation, and Management. Am Fam Physician. 2013; 88: 835–40.

Filho DW, Torres MA, Crezcynski-pasa B, Boveris A. Spermatic cord torsion, reactive oxygen and nitrogen species and ischemia–reperfusion injury. Mol Aspects Med. 2004; 25: 199–210.

Aktoz T, Kanter M, Aktas C. Protective effects of quercetin on testicular torsion/ detorsion-induced ischaemia-reperfusion injury in rats. Andrologia; 2010. p. 376–83.

Dokmeci D, Inan M, Basaran UN, Yalcin O, Aydogdu N, Turan FN, et al. Protective effect of L-carnitine on testicular ischaemia-reperfusion injury in rats. Cell Biochem Funct. 2007; 25(8): 611–8.

Turrens JF. Superoxide Production by the Mitochondrial Respiratory Chain. Biosci Rep. 1997; 17(1): 3–8.

Akondi BR, Challa SR, Akula A. Protective Effects of Rutin and Naringin in Testicular Ischemia - Reperfusion Induced Oxidative Stress in Rats. J Reprod Infertil. 2011; 12(2): 209–14.

Turner TT, Brown K. Spermatic Cord Torsion: Loss of Spermatogenesis Despite Return of Blood Flow. Biol Reprod. 1993; 407: 401–7.

Mogilner J, Elenberg Y, Lurie M, Shiloni E, Coran A, Sukhotnik I. Effect of dexamethasone on germ cell apoptosis in the contralateral testis after testicular ischemia - reperfusion injury in the rat. Fertil Steril. 2006; 85: 1111–7.

Fouad A, El-Bidawy M, Uddin A, Yacoubi M. A preliminary study of dexamethasone against ischemia/reperfusion liver injury in rats. Int J Pharmacol. 2009; 5(2): 155–61.

Yazawa H, Sasagawa I, Suzuki Y, Nakada T. Glucocorticoid hormone can suppress apoptosis of rat testicular germ cells induced by testicular ischemia. Fertil Steril. 2001; 75(5): 980–5.

Copyright Information
Department of Urology, Faculty of Medicine/Airlangga University