EFFECT OF ERYTHROPOIETIN ADMINISTRATION ON THE AMOUNT OF SPERMATOGONIUM, SERTOLI CELL, AND LEYDIG CELL ON RATS TESTIS (WISTAR STRAIN) AFTER VAS DEFERENS LIGATION RELEASED
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Abstract
Objective: To determine the effect of Erythropoietin (EPO) on the number of spermatogonia, Sertoli cells, and Leydig cells in white rats wistar strain testis after the release of ligation vas deferens. Material & Methods: Twenty-four Wistar strain rats were grouped into 4 groups. The control group only performed an orchiectomy for testicular examination, ligation group vas deferens only, group performed release ligation of vas deferens, and group performed release ligation of vas deferens and given EPO injection with dose of 1000 iu/kg BW intraperitoneally for 1 week (3x/week). Observation of spermatogonium, Sertoli cells and Leydig cells by counting the amount on the 5 cross sections of the seminiferous tubules using a 400x light magnification microscope with Haematoxylin Eosin staining. Results: Ligation of vas deferens can significantly decreased the number of spermatogonia and Sertoli cells (p<0.05). In Leydig cells there was no significant difference in numbers after ligation of vas deferens (p>0.05). Release of vas deferens ligation turned out to be no significant amount difference in spermatogonia, Sertoli cells, and Leydig cells with ligation of vas deferens group. Similarly, the treatment of ligation vas deferens release and an EPO injection for 1 week was also no significant difference in number compared to the ligation release group of vas deferens. Conclusion: The number of Sertoli cells, Leydig cells, and spermatogonia in the ligation release group of vas deferens and given EPO for 1 week had the same number with the ligation release group vas deferens.
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Vasectomy reversal, reactive oxygen species, erythropoietin, spermatogonium, Sertoli cell, Leydig cell
Dohle GR, Diemer T, Kopa Z, Krausz C, Giwercman A, Jungwirth A. European association of urology guidelines on vasectomy. Eur Urol. 2012; 61(1):159–63.
Zini A, Grantmyre J, Chan P. CUA guideline: Vasectomy. J Can Urol Assoc. 2016; 10(7–8AUGUST): E274–8.
Frcsgias RGM, Frcs JCB. Reversal of vasectomy using a macroscopic technique: a retrospective study; 1997. p. 420–2.
A Jungwirth, T Diemer, Z Kopa, C Krausz, H Tournaye. Male infertility. EAU Guidelines; 2017.
Kolettis PN, Sharma RK, Pasqualotto FF, Nelson D, Thomas AJJ, Agarwal A. Effect of seminal oxidative stress on fertility after vasectomy reversal. Fertil Steril. 1999; 71(2): 249–55.
Liu J, Rong CT, Li Y, Liu XX, Wang WT, Li N. Vasectomy induces oxidative stress and up-regulates the expression of peroxiredoxins in mouse testis in short and early periods after surgery. J Urol [Internet]. 2014; 191(6): 1920–6. Available from: http://dx.doi.org/10.1016/j.juro.2014.01.007
Duru FIO, Ajayi S, Azu OO. The effect of unilateral vasectomy on testosterone and testicular parameters in the adult male African giant rat (Cricetomys gambianus). Afr Health Sci. 2013; 13(2): 483–9.
Bernie AM, Osterberg EC, Stahl PJ, Ramasamy R, Goldstein M. Vasectomy reversal in humans. Spermatogenesis [Internet]. 2012; 2(4): 273–8. Available from: http://www.tandfonline.com/doi/abs/10.4161/spmg.22591.
Shapiro RH, Muller CH, Chen G, Berger RE. Vasectomy reversal associated with increased reactive oxygen species production by seminal fluid leukocytes and sperm. J Urol [Internet]. 1998; (August 2014): 1341–6. Available from: http://gateway.ovid.com/ovidweb.cgi?T=JS&PAGE=crossref&AN=00005392-199810000-00034.
Alpcan S, Başar H, Aydos TR, Kul O, Kısa Ü, Başar MM. Apoptosis in testicular tissue of rats after vasectomy: Evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy. Turk Urol Derg. 2014; 40(4): 199–206.
Flickinger CJ, Herr JC, Howards SS, Caloras D, Yarbro ES, Spell DR, et al. The influence of vasovasostomy on testicular alterations after vasectomy in Lewis rats. Anat Rec. 1987; 217(2): 137–45.
Gupta AS, Kothari LK, Bapna RB. Surgical sterilization by vasectomy and its effects on the structure and function of the testis in man. Br J Surg. 1975; 62: 59.
Yazihan N, Ataoglu H, Koku N, Erdemli E, Sargin AK. Protective role of erythropoietin during testicular torsion of the rats. World J Urol. 2007; 25(5): 531–6.
Rashed FK, Ghasemi B, Deldade Mogaddam H, Mesgari M. The effect of erythropoietin on ischemia/reperfusion injury after testicular torsion/detorsion: a randomized experimental study. ISRN Urol [Internet]. 2013; 2013: 351309. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23710369.
Weiss MJ. New insights into erythropoietin and epoetin alfa: mechanisms of action, target tissues, and clinical applications. Oncologist [Internet]. 2003; 8(90003): 18–29. Available from: http://theoncologist.alphamedpress.org/content/8/suppl_3/18.full.
Köseoğlu B, Yılmaz E, Ceylan K, Uzun E, Bayram İ, Hızlı F. The protective effect of erythropoietin infusion on testicular torsion/detorsion: an experimental study. Int Urol Nephrol [Internet]. 2009; 41(1): 85–91. Available from: http://link.springer.com/10.1007/s11255-008-9418-8
Hamed MG, Ahmed RM, Maher M MM. Effect of erythropoietin on experimental unilateral testicular torsion detorsion in rat model. Egypt; 2011.
Dahlan, Sopiyudin. Statistik untuk kedokteran dan kesehatan: uji hipotesis. Jakarta: Bina Mitra Press; 2006.
Zhang Y, Wang L, Dey S, Alnaeeli M, Suresh S, Rogers H, et al. Erythropoietin action in stress response, tissue maintenance and metabolism. Int J Mol Sci. 2014; 15(6): 10296–333.
RK Sharma, FF Pasqualotto, H Kobayashi, AJ Thomas, J and AA. Oxidative stress and IL-6 levels in patients undergoing vasectomy reversal. Cent Adv Res Hum Reprod Infertil Dep Urol Clevel Clin Found: Cleveland OH; 2005. p. 89.
Sharma AAR, Harlev SGA, Plessis GASS du, Wang SCESM, Durairajanayagam D. Oxidative stress in human reproduction. SIPA editor; 2017.
Agarwal A. Oxidants and antioxidants in human fertility. 2004; 9(3): 187–97.
Turner Labatt. Leydig cell function after experimental testicular torsion despite loss of spermatogenesis. J Andrology; 1995. p. 12–7.
Maiese K, Chong ZZ, Hou J, Shang YC. Erythropoietin and oxidative stress. Curr Neurovasc Res. 2008; 5(2): 125–42.
Temma K, Shimoya K, Hashimoto K, Zhang Q, Koyama M, Murata Y. Detection of erythropoietin in human seminal plasma. Fertil Steril. 2004; 81(Suppl 1): 798–801.