IN VITRO STUDY ON NIGELLA SATIVA AS INHIBITOR OF CALCIUM OXALATE CRYSTALLIZATION
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Abstract
Objective: This study aims to test the efficacy of Nigella Sativa plant as an inhibitor of calcium oxalate crystallization. Material & Methods: Nigella Sativa alcohol extract was made by maceration of 100 grams Nigella seeds which were dried and powdered for 72 hours and then filtered, so as to produce 100 percent Nigella alcohol extract. Then make dilutions of 10 and 50 percent. The precipitation of calcium oxalate at 37 0C and pH 5.7 was studied by measurement of turbidity at 620 nm with and without inhibitor which is Nigella Sativa. Results: Tmax, the slope of nucleation (SN) and slope of aggregation (SA) was measured with control and with inhibitor. With increasing concentration of inhibitor, Tmax progressively increased, whereas SN and SA decreased. The nucleation percentage inhibition ratio was 86.0% and inhibition of crystal aggregation took place by 66.6% with 100% extract. Crystallization was inhibited (growth and aggregation) in a concentration dependent manner. Conclusion: Nigella Sativa is strong inhibitor of calcium oxalate crystallization. However, comprehensive work is needed to establish the results.
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Nigella Sativa, inhibitor, calcium oxalate
Leye A, Jaegar P, Robertson W, Unwin R. Renal stone disease. Medicine. 2007; 35: 415-418.
Anjum MR, Ahmad R, Ghaffar A, Zaidi AI, Twenty-four hours urinary citrate levels in recurrent renal stone formers and healthy controls. Nishtar Medical Journal. 2009; 1.
Hussain M, Lal M, Ali B , Ahmed S, Muzammil R, Hamid R, Hussain Z, Rizvi SAH Urolithiasis in Sindh: A single centre experience with a review of 10,000 cases. J Nephrol Urol Transplant. 1998; 1: 10-13.
Hussain M, Lal M. et al Management of urinary calculi associated with renal failure. J Pak Med Assoc. 1995; 45: 205-208.
Hadjzadeh MA, Rad Ak et al. The preventive effect of N-butanol fraction of Nigella Sativaon ethylene glycol-induced kidney calculi in rats). Pharmacogn Mag. 2011; 7 (28): 338–343.
Benstal A, Ouahrani MR Inhibition of crystallization of calcium oxalate by extraction of tamarix gallica L. Urol Res. 2008; 36: 283-287.
Berg C, Tiselius HG. The effect of pH on the risk of calcium oxalate crystallization in urine. Eur Urol. 1986; 12: 59–61.
Hennequin C, Lalanne V, Daudon M, Lacour B, Drueke T. A new approach to studying inhibitors of calcium oxalate crystal growth. Urol Res. 1993; 21: 101–108.
Finlayson B. Physicochemical aspects of urolithiasis. Kidney Int. 1978; 13: 344–60.
Hess B, Meinhardt L, Giovanoli JP. Simultaneous measurement of calcium oxalate crystal nucleation and aggregation: Impact of various modifiers. Urol Res. 1995; 23(4): 231–238.
Pareta SK, Patra KC, Harwansh. In vitro calcium oxalate crystallization inhibition by Achyranthes indica Linn. Hydroalcoholic Extract: An Approach to Antilithiasis, International Journal of Pharma and Bio Sciences. 2011; 2: 432-437.
Yadav RD, Jain SK, Alok S, Mahor A, Bharte PJ, Jaiswal M. Herbal plants used in the treatment of urolithiasis: a review. IJPSR. 2011; 2: 1412-1420.
Atmani F, Farell G, Lieske JC. Extract from Herniaria hirsute coats calcium oxalate monohydrate crystals and blocks their adhesion to renal epithelial cells. J Urol. 2004; 172: 1510-1514.
Farook NAM, Dameem GAS, et al. Inhibition of Mineralization of Urinary Stone Forming Minerals by Hills Area Fruit. E-Journal of chemistry. 2004; 1: 137-141.
Zhang CY, Wu WH, Lan MN. Antioxidant properties of Polysachharide from the Brown seaweed Sargassum graminifolium (Turn). and its effects on calcium oxalate crystallization. Mar Drugs. 2012; 10: 119-130.
Das I, Gupta S.K, Ansari SA, Pandey VN, Rastogi RP. In vitro inhibition and dissolution of calcium oxalate by edible plant Trianthema monogyna and pulse Macrotyloma uniflorum extracts. J. Cryst. Growth. 2005; 273: 546–554.
Hadjzadeh MA, Khoei A et al. Ethanolic Extract of Nigella Sativa L Seeds on Ethylene Glycol-Induced Kidney Calculi in Rats. Urology Journal. 2007; 4: 86-90.
Hadjzadeh MA, Mohammadian N et alEffect of Thymoquinone on Ethylene Glycol-Induced Kidney Calculi in Rats. Urology Journal. 2008; 5: 149-55.