Published 2021-01-15
Suryo Prasetyo Furqan Hidayatullah Indra Bachtiar Arif Rachman Indri Lakhsmi Putri Fabio Castiglione Doddy M. Soebadi Lukman Hakim


Objective: This study aimed to gives a perspective in CM-ADMSCs effect in urothelial bladder cancer viability. Material & Methods: Human bladder cell carcinoma type 5637 was used as the subject of this in vitro study. This study contains four different groups: untreated control group, Culture medium: hADMSCs with 1:1, 1:2, and 1:4 concentration group. Each group consists of 6 replications to prevent bias of the study. Viability was determined with MTT assay methods and evaluation performed after 48 h exposure of conditioned medium. Results: A post hoc test was conducted to analyze the data. The 5637 bladder cancer cell line demonstrated significantly decreased viability after exposure to culture medium: CM-hADMSCs 1:1 (p: 0.002) compared to the negative control group, but there are no significant differences in viability between the control groups with groups that were exposed to culture medium: CM-hADMSCs 1:2 and culture medium: CM-hADMSCs 1:4 with p: 0.480 and p: 0.060 respectively. Conclusion: Decreased viability of urothelial bladder cancer cells after exposure to CM-hADMSCs occurs at a concentration of 1:1 and Dosage addition more than 1:1 concentration doesn’t give any advantages.



Bladder cancer, conditioned medium, adipose-derived mesenchymal stem cells, viability, MTT


Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019. CA: A Cancer Journal for Clinicians. 2019; 69(1): 7–34.

Umbas R, Safriadi F, Mochtar CA, Djatisoesanto W, Hamid ARAH. Urologic cancer in Indonesia. Japanese Journal of Clinical Oncology. 2015; 45(8): 708–712.

Lee HY, Hong IS. Double-edged sword of mesenchymal stem cells: Cancer-promoting versus therapeutic potential. Cancer Sci. 2017; 108: 939–1946.

Li Z, Fan D, Xiong D. Mesenchymal stem cells as delivery vectors for anti-tumor therapy. Stem Cell Investigation. 2015; 2(6): 6.

Xiang B, Chen L, Wang X, Xiang C. Mesenchymal stem cells as therapeutic agents and in gene delivery for the treatment of glioma. Journal of Zhejiang University-SCIENCE B. 2017; 18(9): 737–746.

Vizoso FJ, Eiro N, Cid S, Schneider J, Perez-Fernandez R. Molecular Sciences Mesenchymal Stem Cell Secretome: Toward Cell-Free Therapeutic Strategies in Regenerative Medicine. J. Mol. Sci. 2017; 18: 2-24.

Rahman A. et al. Biocompatibility of Yttria-Tetragonal Zirconia Polycrystal Seeded with Human Adipose Derived Mesenchymal Stem Cell. Acta Inform Med. 2018; 26(4): 245-248.

Maj M, Bajek A, Nalejska E, Porowinska D, Kloskowski T, Gackowska L, Drewa T. Influence of Mesenchymal Stem Cells Conditioned Media on Proliferation of Urinary Tract Cancer Cell Lines and Their Sensitivity to Ciprofloxacin. Journal of Cellular Biochemistry. 2017; 118(6): 1361–1368.

Otsu K. et al. Concentration-dependent inhibition of angiogenesis by mesenchymal stem cells. 2016; 113(18): 4197–4206.

Hou L. et al. Inhibitory effect and mechanism of mesenchymal stem cells on liver cancer cells. International Society of Oncology and Bio Markers. 2013; 35: 1239–1250.

Ramasamy R. et al. Mesenchymal stem cells inhibit proliferation and apoptosis of tumor cells: impact on in vivo tumor growth. Nature. 2007; 21: 304–310.

Yang C. et al. Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines In Vitro. BioMed Research International: Hindawi Publishing Corporation; 2014. p. 1–13.

Copyright Information
Department of Urology, Faculty of Medicine/Airlangga University