TRANSPLANTATION OF ALLOGENIC ADIPOSE-DERIVED STEM CELL IMPROVE URETHRAL MUSCLE-COLLAGEN RATIO AFTER VAGINAL DISTENSION

##plugins.themes.bootstrap3.article.main##

##plugins.themes.bootstrap3.article.sidebar##

PDF
Published 2014-07-01
I Wayan Yudiana Sunaryo Hardjowijoto Sabilal Alif Purwati Purwati Willy Sandhika Budiono Budiono

Abstract

Objective:To investigate the effects of allogenic adipose-derived stem cell (ADSC) transplantation on urethral tissue regeneration after vaginal distension in a rat model.Material & Method:32 female wistar rats underwent vaginal distension (VD) for 4 h. Subsequently, PKH-labeled ADSCs (2 x 105 in 200 µl PBS, ADSC group, n = 18) or PBS (200 µl, placebo group, n = 18) were injected into periurethral tissue. Two and four weeks following transplantation (ADSC or PBS), voiding behavior (frequency) and muscle-collagen ratio of urethral tissue were measured to evaluate urethral sphincter regeneration. Data were analysed by paired-t test, one-way ANOVA and post hoc LSD.Results:Mean voiding frequency was significantly different in the ADSC group at two weeks (p<0.0001), but not significantly different at four weeks (p=0.448) when compared with the placebo group.Masson’s trichrome staining revealed that the muscle-collagen ratio of urethral tissue was not significantly different between study groups at two or four weeks (p=0.053 or p=0.166 respectively). Muscle-urethral ratio was more specific showing a significant difference at two weeks (p=0.043). There were significant differences about muscle-collagen or muscle-urethral ratio between control and placebo groups (p<0.05), whereas between control and ADSC groups no significant difference was observed (p>0.05).Conclusion:ADSC transplantation promotes urethral muscle-collagen ratio with development of striated muscle after vaginal distension, so that can improve voiding behavior in a rat model.Keywords:Adipose-derived stem cell, vaginal distension, rat-SUI model, muscle-collagen ratio, voiding stain on paper.


##plugins.themes.bootstrap3.article.details##

References

Abrams P, Cardozo L, Fall M, Griffiths D, Rosier P, Ulmsten U, et al. The standardisation of terminology of lower urinary tract function: Report from the Standardisation Sub-committee of the International Continence Society. Neurourology and Urodynamics. 2002; 21: 167-78.

Chapple CR, Milsom I. Urinary incontinence and pelvic prolapse: Epidemiology and Pathophsiology..In: Wein AJ, Kavoussi LR, Novick AC, Partin AW, Peters CA (eds). Campbell-Walsh Urology. 10th ed. Philadelphia: Elsevier-Sounders; 2012. p. 1871-95.

Hunskaar S, Burgio K, Diokno A, Herzog R, Hjalmas K. Avoid this scary MC. Epidemiology and Natural History of Urinary Incontinence in Women. Urology. 2003; 62(4A): 16-23.

Dooley Y, Kenton K, Luke A, Dura-Arvizu R zo. Urinary incontinence prevalence: Results From the National Health and Nutrition Examination Survey. The Uro J. 2008; 179: 656-61.

Hansen BB, Svare J, Viktrup L, Jorgensen T, Lose G. Urinary incontinence during pregnancy and 1 year after delivery in primiparous women compared with a control group of nulliparous women. Neurourol Urodyn. 2012; 31: 475-80.

Payne CK. Conservative management of urinary incontinence: Behavioral and pelvic floor therapy, urethral and pelvic devices. In: Wein AJ, Kavoussi LR, Novick AC, Partin AW, Peters CA (eds). Campbell-Walsh Urology. 10th ed. Philadelphia: Elsevier-Sounders; 2012. p. 2004-25.

Chappel CR. Retropubic suspension surgery for incontinence in women. In: Wein AJ, Kavoussi LR, Novick AC, Partin AW, Peters CA (eds). Campbell-Walsh Urology. 10th ed. Philadelphia: Elsevier-Sounders; 2012. p. 2047-68.

Schroder A, Abrams P, Andersson KE, Artibani W, Chapple CR, Drake MJ, et al. Guidelines on Urinary Incontinence. In: Parson KF, Irani J, Hanus T, Loch T, Llorente C, Mitrop oulos D (board members). EAU Guidelines 2012. European Association of Urology; 2012. p. 4-52.

Halim D, Murti H, Ferry S. Stem cell: Basic theory and clinical application. New York: McGraw; 2010.

Cannon TW, Lee JY, Somogyi G, Pruchnic R, Smith CP, Huard J, et al. Improved sphincter contractility after allogenic progenitor muscle-derived cell injection into the denervated rat urethra. Urology. 2003; 62: 958-63.

Fu Q, Song XF, Liao GL, Deng CL, Cui L. Differentiated myoblasts from adipose-derived stem cells to treat stress urinary incontinence. J Urol. 2009; 75: 718-23.

Lin G, Wang G, Banie L, Ning H, Shindel AW. Treatment of stress urinary incontinence with adipose tissue derived stem cells. Cytotherapy. 2010; 1: 88-95.

Zhao W, Zhang C, Jin C, Zhang C, Kong D, Xu W, et al. Periurethral injection of autologous adipose-derived stem cells with controlled-release nerve growth factor for the treatment of stress urinary incontinence in a rat model. EuroUrol. 2011; 59: 155-63.

Mitterberger M, Pinggera GM, Marksteiner R, Margreite RE, Fussenegger M. Adult stem cell therapy of female stress urinary incontinence. Euro Uro. 2008; 53: 169-75.

Carr LK, Steele D, Steele S, Wagner D. 1-year follow-up of autologous muscle-derived stem cell injection pilot study to treat stress urinary incontinence. Urogynecol Int J. 2008; 19: 881-3.

Pan HQ, Kerns JM, Esparza N, Damaser MS. Increased duration of simulated child birth injuries results in increased time to recovery. Am J Physiol Regul Integr Comp Physiol. 2007; 292: 1738-44.

Prantil RL, Jankowski RJ, Kalho Y, de Groat WC, Chancellor MB, Yoshimura N, et al. Ex vivo biomechanical properties of the female urethra in a rat model of birth trauma. Am J Physiol Renal Physiol. 2006: 292: 1229-37.

Sugino Y, Kanematsu A, Hayashi, Haga H, Yoshimura N. Voided stain on paper method for analysis of mouse urination. Neurourol & Urodynam. 2008; 7: 548-52.

Heidkamp MC, Leong FC, Brubaker L, Russell B. Pudendal denervation affects the structure and function of the striated urethral sphincter in female rats. Urogynecol Int J. 1998; 9: 88-93.

Yamamoto N, Akamatsu H, Hasegawa S, Yamada T, Nakata S. Isolation of stem cells from mouse multipotent adipose tissue. Journal of Dermatological Science. 2007; 48: 43-52.

Damaser MS, Whitbeck C, Chichester P, Levin RM. Effect of vaginal distension on blood flow and hypoxia of urogenital organs of the female rat. J Appl Physiol. 2005; 98: 1884-90.

Damaser MS, Broxton-King C, Ferguson C, Kim FJ, JM Kerns. Functional and neuroanatomical effects of vaginal distention and pudendal nerve crush in the female rat. Uro J. 2003; 170: 1027-31.

Lin AS, Carrier S, Morgan DM, Lue TF. Effect of simulated birth trauma on urinary continence mechanism in the rat. Urology. 1998; 52: 143-51.

Section
Articles
Copyright Information
Department of Urology, Faculty of Medicine/Airlangga University